Abstract
Feto-placental unit requires large amounts of nucleotides and nucleic acids due to its rapid growth and metabolic rate. We studied purine nucleotide synthesis in a highly enriched population of trophoblastic cells from normal first and third trimester placentae, obtained with collagenase digestion and density gradient. De novo synthesis was measured as incorporation of 14C-formate and reutilization as incorporation of 14C-adenine (Ade), -hypoxanthine (Hx), and -adenosine (Ado). Incorporation of formate was significantly (p<0.01) less in third trimester cells. Ade incorporation was an order of magnitude higher than that of formate, and significantly (p<0.001) higher in first than third trimester cells. Hx incorporation did not change as a function of gestational age. High (10 mM) extracellular inorganic phosphate did not enhance Hx phosphoribosylation. Both Ado phosphorylation and deamination increased with concentration. High Ado (60 uM) was more efficiently utilized in first trimester cells. Concl.: 1) major pathways of purine nucleotide synthesis are functional in human trophoblast throughout gestation, 2) contribution of reutilization to the synthesis appears larger than that of de novo pathway, 3) rate of nucleotide and nucleic acid synthesis decreases with gestational age, 4) hypoxanthine may be the major precursor utilized in trophoblastic purine nucleotide synthesis.
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K, V., Raivio, K. 66 PURINE NUCLEOTIDE SUPPLY OF THE HUMAN TROPHOBLAST. Pediatr Res 24, 271 (1988). https://doi.org/10.1203/00006450-198808000-00092
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DOI: https://doi.org/10.1203/00006450-198808000-00092