Abstract
Gut hormones were shown to affect the digestive process. However, little is known about their interaction with the immune system. We investigated the effect of CCK-OP on 3H-thymidine incorporation into rat splenic (SL) and peripheral blood lymphocytes (PBL) induced by Con-A. Various doses of CCK-OP (10−8M to 10−14M) were incubated with Con-A (33μg/ml) and lymphocytes in cell cultures (2X105 cells/well) for 72 hrs; 3H-thymidine (0.4 μci/well) was added and cultures harvested 18 hrs. later. Incorporation of thymidine was quantitated in a Beckmann Liquid Scintillation System (Irvine, Ca). The results (Table) suggest an enhancement of Con-A-induced lymphoproliferative response of spleen cells by up to 48%. In contrast, PBL did not show any effect of CCK-OP on Con-A induced cell proliferation. CCK-OP alone did not induce lymphoproliferation.
These data suggest that physiological concentrations of CCK-OP enhance lymphocyte proliferation and thus may partake in the regulation of the immune response.
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Elitsur, Y., Albini, B. & Lebenthal, E. CHOLECYSTOKININ OCTAPEPTID (CCK-OP) ENHANCES THE PROLIFERATIVE RESPONSE OF RAT SPLENIC LYMPHOCYTES TO CONCAVALIN A (CON-A). Pediatr Res 21 (Suppl 4), 311 (1987). https://doi.org/10.1203/00006450-198704010-00861
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DOI: https://doi.org/10.1203/00006450-198704010-00861