Abstract
In the past, dexamethasone and glucagon has been shown to have a variable effect on the expression of soluble liver enzymes during development. The aim of the present study was to investigate the effect of dexamethasone and glucagon on the expression of plasma membrane proteins during ontogeny. Our previous studies have demonstrated a significant increase of the asialoglycoprotein receptor (ASGR) and 110,000 Mr glycoprotein at birth, decrease in alkaline phosphatase (AP) and gammaglutamyltranspeptidase (GT) and very little change in leucine aminopeptidase (LAP) after birth. Buffalo rat fetuses at 18 days of gestation and 1-day-old newborns were injected (single and daily, respectively) with dexamethasone (2 and 4 μg, respectively) or glucagon (25 and 50 μg, respectively). Fetuses were sacrificed at 22 days of gestation and newborns at 3, 5, and 7 days of age. Total post-nuclei membrane vesicles were prepared from rat livers using a sucrose gradient method. Quantitation of levels of membrane proteins was performed using immunological and/or enzymatic methods. In fetal rats, dexamethasone did not affect the expression of membrane proteins. In contrast, glucagon increased significantly the levels of AP, LAP, and GT. In newborns, glucagon did not alter membrane protein levels. However, dexamethasone dramatically increased six-fold the level of GT while the amounts of ASGR, AP, and LAP were reduced by two-to four-fold. These data indicate that there is a differential effect of dexamethasone and glucagon on the expression of membrane proteins during late intrauterine and early extrauterine stages of development.
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Bujanover, Y., Ammari, S., Lebenthal, E. et al. HORMONAL REGULATION OF LIVER PLASMA MEMBRANE PROTEIN EXPRESSION DURING DEVELOPMENT. Pediatr Res 21 (Suppl 4), 265 (1987). https://doi.org/10.1203/00006450-198704010-00585
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DOI: https://doi.org/10.1203/00006450-198704010-00585