Abstract
Aerosol challenge of sensitized rats leads to an apparent increase in permeability of the fixed trachea (Clin Exp Immunol 1986; 65:647). The present study was undertaken to show that this increased permeabilty occurs Because of increased porosity of the luminal surface in life. Rats were sensitized with DNP19 ovalbumin (DNP-OA) or saline. There were exposed to an aerosol of DNP-OA plus lanthanum nitrate for 1 hour. Tracheas were processed immediately after challenge. The elemental lanthanum concentration was measured oy X-ray microanalysis with KeVex 5,000 A/6,000 spectrometers. The lanthanum penetrated Between the cells. Its concentration was greatest at the airway surface and decreased progressively towards the eartilage. More lanthanum was present in the epithelium of 6 DNP-OA sensitized rats (42.9 ±6.0 counts/100 sec) compared to 6 controls (11.9 ±1.5 counts/100 sec), (P<0.01, Mann-Whitney). The DNP-OA sensitised group showed a more severe response on body plethysmography. There is an increase in permeability of the luminal surface of the trachea on challenge with aerosol antigen, prooably as a result of the opening of the intercellular junctions. (Wellcome Trust Supported).
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Carswell, F., Mukherjee, S. & Heap, P. Aerosol Challenge of Sensitised Rats increases the Permeability from the Lumen in Life. Pediatr Res 22, 224 (1987). https://doi.org/10.1203/00006450-198708000-00066
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DOI: https://doi.org/10.1203/00006450-198708000-00066