Abstract
VC has been shown to improve chemotaxis in normal newborns. We evaluated its effect in vitro on 11 sick term NBs, including 8 on antibiotics for undocumented sepsis, on day 2-3 of life. WBCs, separated from the blood, were divided into 2 aliquots. One was incubated for 30 min with Hank's solution (HS) containing 20 μg/ml VC and the other with HS alone.
In a separate study 10 different term NBs with suspected sepsis were treated with 4 doses of lOOmgs of VC q6h starting on day 2 of life. Blood was drawn before and after VC treatment. Chemotaxis was determined by a modified Boyden's chamber technique. PMNs were deposited on a 3μ millipore filter which divided the chamber into an upper compartment filled with Hank's solution (HS), and lower filled with a mixture containing AB serum, endotoxin and HS. Following incubation and staining a ratio of migrated cells to total cells was determined (CI). In a parallel run with HS on either side the ratio was termed RM.
Mean CI increased 64% following VC incubation and VC therapy. RM remained unchanged in both studies. VC may be a useful adjunct to therapy of NBs with sepsis but more studies are needed.
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Vohra, K., Khan, A., Rosenfeld, W. et al. 421 EFFECT OF IN VITRO AND SYSTEMIC VITAMIN C (VC) ON THE NEUTROPHIL FUNCTION IN SICK NEWBORNS (NBs). Pediatr Res 19, 181 (1985). https://doi.org/10.1203/00006450-198504000-00451
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DOI: https://doi.org/10.1203/00006450-198504000-00451