Abstract
Peripheral T-lymphocyte populations from two Lesch-Nyhan heterozygotes were found to consist of 95% hypoxanthineguanine phosphoribosyltransferase (HGPRT)-positive and 5% negative cells by the culture of the T-cells with 6-thioguanine. The T-cells frome each heterozygote were cultured in the conditions in which either enzyme-positive (azaserine-hypoxanthine medium) or negative (6-thioguanine medium) cells selectively proliferate. After two weeks, each separated T-cell subpopulation was considered to be pure with regard to the enzyme expression. Bewteen the enzyme-positive and negative subpopulations, percentages of OKT3, OKT4 or OKT8-positive T-cells were compared. The fact that only small percentage of peripheral T-cells are HGPRT-negative in the heterozygotes suggests that in vivo differentiation of HGPRT-negative cells is disturbed in the heterozygotes. Our data have shown that both enzyme-positive and negative T-cell subpopulations from each heterozygote possessed equivalent percentages of OKT4, OKT8 and OKT3-positive cells. These results suggest that, in the heterozygotes, growth or differentiation of HGPRT-nnegative lympoid cells of T-cell linkage was disturbed before OKT4 and OKT8-bearing thymocyte subpopulations separate from each other.
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Yamanaka, H., Nishioka, K., Mori, M. et al. COMPARISON OF SURFACE DIFFERENTIATION ANTIGENS BETWEEN SEPARATED HGPRT-POSITIVE AND NEGATIVE T-CELL POPULATIONS FROM TWO LESCH-NYHAN HETEROZYGOTES: 233. Pediatr Res 19, 782 (1985). https://doi.org/10.1203/00006450-198507000-00253
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DOI: https://doi.org/10.1203/00006450-198507000-00253