Abstract
Nucleic acid synthesis was measured in nuclei isolated from proliferating lymphoblast cell lines that had been incubated with deoxyadenosine (dAdo) plus deoxycoformycin (dCf). Both RNA and DNA synthesis were inhibited in nuclei that were isolated from a T-cell line, CEM, following treatment with dAdo and dCf. Conversely, there was no inhibition of either RNA or DNA synthesis in the nuclei isolated from a B-cell line, WI-L2, or a dAdo kinase-deficient mutant of the T-cell line which had been incubated with dAdo plus dCf. Nucleotide measurements in the three cell lines implied that the accumulation of dATP in the intact cell was necessary for the inhibition of nucleic acid synthesis in the isolated nuclei. Saturating concentrations of all the nucleoside triphosphate. precursors were present in the assays of the isolated nuclei. Thus, the inhibition of nucleic acid synthesis could not be explained by a depletion of nucleotide precursors following an inhibition of ribonucleotide reductase by dATP. After the isolation and washing procedure, no endogenous nucleotides could be detected in the isolated nuclei. The accumulation of dATP in the intact cell apparently damaged the ability of isolated nuclei to synthesize nucleic acids and this damage persisted even in the absence of dATP.
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Matsumoto, S., Yu, A. ROLE OF dATP THE INHIBITION OF NUCLEIC ACID SYNTHESIS IN ISOLATED NUCLEI: 129. Pediatr Res 19, 765 (1985). https://doi.org/10.1203/00006450-198507000-00149
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DOI: https://doi.org/10.1203/00006450-198507000-00149