Abstract
In tissues with a high rate of fatty acid utilization, intra-cellular free fatty acids are bound to a low molecular weight (12000 daltons) fatty acid binding protein (FABP). By differential binding of fatty acids, FABP may compartmentalize different fatty acid species and regulate cellular free fatty acid utilization. To determine if lung contains cytosolic proteins that bind free fatty acids, we prepared a 100,000xg supernatant of whole rabbit lung and incubated it with radiolabeled palmitate. After gel filtration on a calibrated Sephadex G-75 column, two peaks of radioactivity were noted. The first eluted in the void volume and probably represents high molecular weight aggregates of fatty acids. A second peak eluted at the volume calibrated to be approximately 12000 daltons. Similar studies on supernatants prepared from pure adult type II pneumocytes showed a similar elution profile. No peak in this region was observed following incubation of the cytosol with radiolabeled phospha-tidylcholine. Incubation with equimolar concentrations of radiolabeled oleate produced a smaller peak than observed with palmitate. These studies suggest that whole lung and pure type II cells contain an intracellular binding protein for fatty acids which may be similar to FABP. (Supported by a grant from the March of Dimes--Birth Defects Foundation 1–923).
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Maniscalco, W., Finkelstein, J. 1793 FATTY ACID BINDING TO LUNG CYTOSOLIC PROTEINS. Pediatr Res 19, 409 (1985). https://doi.org/10.1203/00006450-198504000-01811
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DOI: https://doi.org/10.1203/00006450-198504000-01811