Abstract
A panel of monoclonal antibodies (McAb) prepared against surface antigens of freshly isolated adult rabbit type II pneumocytes was used to investigate the appearance of type II pneumocyte specific populations in fetal rabbit lung. Antibody binding to cells was assessed both by immunoperoxidase assay as well as indirect immunofluorescence detection with avidin conjugated phycoerythrin (PE). Fluorescent cells were analyzed by laser flow cytometry to identify specific cellular populations at various fetal ages. Polyacrylamide gel electrophoresis and immunoblot analysis were used to identify specific antigenic species on the cells. In adult lung cell populations a McAb (API63) exhibited prominent staining of lamellar body (LB) containing cells. API63 also showed high staining of LB-containing cells from fetuses at 27 day and 28 day gestation. Moreover, API63 exhibited a distinct antibody positive subpopulation in cells from 24 day fetuses, where LB containing cells are negligible. Two additional McAbs, API117 and API125 were also positive for LB containing cells in adults but showed progressive decline with decreasing gestational age. These results suggest that different patterns of expression of cell surface antigens are present in the developing lung. Combinations of McAb bound to specific antigens may be useful in isolation of undifferentiated precursors of type II pneumocytes. (Supported by NSF Grant PCM8215152 and NIH Grant HL 29915)
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Finkelstein, J., Leary, J., Notter, R. et al. 1758 IDENTIFICATION OF POPULATIONS OF CELLS FROM FETAL RABBIT LUNG USING MONOCLONAL ANTIBODIES AGAINST ADULT TYPE II PNEUMOCYTES. Pediatr Res 19, 403 (1985). https://doi.org/10.1203/00006450-198504000-01776
Issue Date:
DOI: https://doi.org/10.1203/00006450-198504000-01776