Abstract
We determined the rate of ATP depletion and catabolism in 8 canine immature hearts (IM-2 weeks old) and 5 adult (AM) hearts in response to normothermic global ischemia in vitro. Transmural serial biopsies were obtained just before ischemia and at 2, 5, 10, 15, 20, 25, 30, 40, 50 and 60 min of ischemia. Myocardlal adenine nucleotides, nucleosides, purines and NAD+ were determined by HPLC.
IM retained more AMP than AM during ischemia. Purines accumulated more in AM than IM. IM may have either less 5′-nucleotidase and/or the enzyme may be kinetically less responsive to AMP accumulation. AMP reserve in ischemic IM may enhance the tolerance of newborn hearts to ischemia and hypoxia by increasing the rate of ATP repletion bypassing both the salvage and the de novo pathways. Inhibition of 5′-nucleotidase may provide increased metabolic protection in mature hearts during ischemia and reperfusion.
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Abd-Elfattah, A., Godwin, C., McRae, R. et al. 70 BIOCHEMICAL BASES FOR TOLERANCE OF THE NEWBORN HEART TO ISCHEMIC INJURY: DEVELOPMENTAL DIFFERENCES IN ADENINE NUCLEOTIDE DEGRADATION BETWEEN ISCHEMIC IMMATURE AND ADULT MYOCARDIUM. A POSSIBLE ROLE OF SARCOLEMMAL 5′-NUCLEOTIDASE. Pediatr Res 19, 122 (1985). https://doi.org/10.1203/00006450-198504000-00100
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DOI: https://doi.org/10.1203/00006450-198504000-00100