Intravenous (IV) hyperalimentation has improved the survival and outcome of the LBW infant, however, long-term placement of IV catheters has been associated with the development of catheter related sepsis. Fibronectin, a large glycoprotein (MW 440,000), has a binding site for Staphylococcal protein A and modulates bacterial adherence to mucous membranes in vivo. We determined the in vitro adherence of labelled (H3-leucine) coagulase positive (CPS) (N=3 strains) and coagulase negative (CNS) (N=3 strains) staphylococcus to PVC and HBP catheters incubated at 37°C in phosphate-buffered saline (pH 7.t). Bacterial adherence was measured at five intervals (0.25, 0.5, 1, 2, 4 hours). Similar experiments were performed following PVC and HBP catheter preincubation with 0.5 ug/ml FN. In addition, we determined the binding of C14-labelled purified FN to PVC and HBP catheters in vitro. After four hours of incubation, PVC catheters bound significantly more CNS than CPS (p < 0.01), while HBP catheters bound significantly more CPS than CNS (p < 0.01). FN significantly increased adherence of CPS to PVC (p < 0.01) catheters and CNS to HBP (p< 0.01) catheters. PVC catheters bound significantly more FN (p < 0.01) than HBP catheters. In summary, 1) the adherence of staphylococci to commonly used indwelling catheters appears species specific and 2) Staphylococcal adherence to IV catheters may be enhanced by fibronectin. Catheter composition and its bacterial adherence characteristics may influence the spectrum of nosocomial pathogens to which the infant is susceptible.
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Russell, P., Kline, J., Yoder, M. et al. 1157 STAPHYLOCOCCAL ADHERENCE TO POLYVINYL CHLORIDE (PVC) AND HEPARIN-BONDED POLYURETHANE (HBP) CATHETERS IS SPECIES SPECIFIC AND IS ENHANCED BY FIBRONECTIN (FN). Pediatr Res 19, 303 (1985) doi:10.1203/00006450-198504000-01187