Abstract
The morbidity and mortality associated with neonatal EC infection have remained significant despite advances in antimicrobial chemotherapy. In a search for more effective therapy, we evaluated combination therapy against a K1 EC with cefotaxime (Ct) and two different antibody (Ab) preparations, rabbit J5 antiserum (J5 Ab) and murine IgM monoclonal antibody to the lipopolysaccharide of the infecting EC serotype (LPS Ab). EC bacteremia and meningitis.were induced in 5-day-old rats by sc inoculation. At 6 days of age, each litter was divided into 3 groups to receive Ct (50 mg/kg sc twice daily), one of the Ab preparations (50 μl/10 gm ip) or combination therapy. A control rat from each litter received saline. Bacterial counts in blood and CSF were determined daily before and during therapy and mortality recorded. All animals receiving saline died. Bacterial clearance was inversely related to the mortality, which is summarized below:
As noted, LPS Ab enhanced the efficacy of Ct while J5 Ab was not beneficial. These findings suggest that the combination of antibiotics and LPS Ab have enhanced efficacy in this model.
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Kim, K., Wass, C., Ziegler, E. et al. 1122 TREATMENT OF EXPERIMENTAL E. COLI (EC) INFECTION WITH THE COMBINATION OF CHEMOTHERAPY AND IMMUNOTHERAPY. Pediatr Res 19, 297 (1985). https://doi.org/10.1203/00006450-198504000-01152
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DOI: https://doi.org/10.1203/00006450-198504000-01152