Abstract
The frequent inclusion of heparin in fluids used for total parenteral nutrition in infants prompted an investigation of the ability of heparin to release lipoprotein lipase (LPL) and hepatic lipase (HL) from the endothelial surface into the circulation and of the effect of heparin on tissue stores of lipase in the postnatal period. In rat pups, plasma post heparin lipolytic activity (PHLA) released by I.P. administration of 0.5 U/g body wt was 15% of adult values at birth and increased rapidly to reach 60% on day 10. Repeated doses of heparin (in adult rats, given 0.1 U/g I.V.) at 1 and 4 hr after the initial dose did not affect the maximal response to heparin. In all age groups 80% of PHLA was inhibited by .5 M NaCl, suggesting a mostly non-hepatic origin for the released enzyme. Heart, lung and liver lipase activities of rat pups were not significantly different from controls not given heparin. The pattern of change in tissue enzyme content was different for heart and lung in which LPL activity increased from 10 and 30% to 60 and 100% of adult values between birth and 10 days and for the hepatic enzyme which exceeded adult levels at birth and decreased to 50% of adult values during the latter half of the suckling period (day 10-21). Our results demonstrate that heparin does not cause depletion of tissue lipases in the postnatal period. The parallel increases in LPL content of peripheral tissues and PHLA suggest that in all age groups heparin induced release of LPL into the circulation is proportional to tissue lipolytic activity. (Support NIH Grant HD-15631).
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Zaidan, H., Gutman, A., Berkow, S. et al. EFFECT OF HEPARIN ON SERUM AND TISSUE LIPASES IN THE DEVELOPING RAT. Pediatr Res 18 (Suppl 4), 148 (1984). https://doi.org/10.1203/00006450-198404001-00329
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DOI: https://doi.org/10.1203/00006450-198404001-00329