Abstract
Littermate Swiss-Webster mouse pups were divided into control and T3 treated (500 ng sc/d × 5d) groups. Following sacrifice on the 5th day, dorsal skins were dissected and floated for 90 min in phosphate buffered saline (PBS), pH 7.3, containing .01M dithiothreitol. Epidermal sheets were separated, homogenized and assayed for EGF by homologous RIA. T3 treatment increased EGF levels (307±25 vs 159±10 pg EGF/mg Lowry protein; mean±SEM, p< .01). Circular sections (area 0.7 cm2) of dorsal skin were suspended in 400 μl PBS+2.5% glucose containing 200,000 cpm I125-EGF. Analysis of binding curves showed increased uptake of label in skin of T3-treated pups after 30 and 60 min incubation (1.26 and 1.27 × controls (p<.01). Binding data were normalized per mg wet weight of skin. Membranes were prepared from homogenate supernatants by centrifugation (2 spins for 30 min at 40,000 g) with resuspension of pellets in PBS+0.5% BSA. 75-125 μg membrane protein were incubated for 30 min with I125-EGF. There was increased binding in the T3 group after passage of membranes over millipore filters (1.40 × control, p<.03). Summary and conclusions: 1) In vivo T3 treatment elevates EGF levels in neonatal mouse epidermis. 2) T3 increases I125-EGF binding to whole skin in organ culture and to crude skin cell membrane preparations. 3) The increase in skin EGF induced by T3 may be due to T3 induction of EGF receptor binding.
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Hoath, S., Lakshmanan, J. & Fisher, D. EFFECT OF TRIIODOTHYRONINE ON EPIDERMAL GROWTH FACTOR CONCENTRATION IN NEONATAL MOUSE EPIDERMIS AND BINDING KINETICS IN WHOLE SKIN ORGAN CULTURE AND MEMBRANE PREPARATIONS. Pediatr Res 18 (Suppl 4), 139 (1984). https://doi.org/10.1203/00006450-198404001-00277
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DOI: https://doi.org/10.1203/00006450-198404001-00277