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Action of androgen on fibroblast collagen synthesis: a receptor-dependent response.


Several groups are still investigating an "in vitro" biological response of androgen action. The purpose of this study was to determine whether dihydrotestosterone (DHT) influences the secretion of collagen by cultured foreskin fibroblasts raised from patients with normal or undetectable DHT-binding activity (complete androgen resistance). Collagen synthesis and secretion were evaluated by 3H-proline incorporation in newly synthetized collagen by confluent fibroblasts alone, or in the presence of DHT (10−5 to -10−10M). Production of collagen is expressed as a ratio between tritiated OH-proline and OH-proline plus proline,within the cells or in the culture medium.

These data show that : -in control cells,DHT significantly increases production of collagen released in the culture medium (p < 0.001), -In complete androgen resistance with no detectable androgen binding activity, DHT does not modify basal collagen synthesis. These results strongly suggest that this hormonal response is mediated via a DHT-receptor or is receptor-dependent. This could be used as a biological response of androgen action in patients with androgen resistance associated with a "post-receptor" defect.

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Loire, C., Kern, B. & Sultan, C. Action of androgen on fibroblast collagen synthesis: a receptor-dependent response.. Pediatr Res 18, 1230 (1984).

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