Abstract
Within recent years different test systems have been developed for EPO determination basing on either its biologic(b)activity or its immunologic (i)criteria. To eludicate the relationship of these two test principles an in-vitro bioassay, measuring 59-Fe incorporation in hematologically active liver cells of 15 days old fetal mice, was compared with a hemagglutination inhibition test.Only b-EPO,significantly(r=-0.96) correlated with hemoglobin(Hb)concentration in normal and anemic children without renal failure(n=30). In spite of a greater variation of i-EPO, median values in normal children did not significantly differ from each other in both test systems. In renal failure EPO activity was decreased with mean i-EPO higher than b-EPO,only the latter,however,significantly correlating with Hb and its half saturation pressure in contrast to i-EPO. Accordingly no relationship between b-EPO and i-EPO could be detected(r=0.08). Data suggest that b-EPO,as measured by the fetal mouse liver cell test, determines biologically valid EPO concentration, whereas i-EPO,as measured by the hemagglutination inhibition test, additionally detects inactive EPO fragments especially accumulating in renal failure.
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Müller-wiefel, D., Schönberg, D. & Schärer, K. Biologic and immunologic measurements of serum erythropoietin (EPO) in children. Pediatr Res 18, 1224 (1984). https://doi.org/10.1203/00006450-198411000-00143
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DOI: https://doi.org/10.1203/00006450-198411000-00143