Abstract
Liquid ventilation (LV) with oxygenated fluorocarbon (RIMAR 101R) was utilized to develop an extrauterine preterm lamb model. Fifteen preterm lambs ranging in age from 106 to 142 days gestation (0.70 to 0.95 gestation) were studied. At elective cesarean section, the fetal carotid artery, jugular vein and trachea were cannulated. The umbilical cord was clamped and the lamb was liquid ventilated through the tracheostomy. Controlled LV was delivered over 2 hrs at an FRC = 30 ml/kg; tidal volume = 15 ml/kg; frequency = 5-9 brth/min. Normothermia was maintained by radiant (heat lamp) and convective (warmed liquid fluorocarbon) heat exchange. Dextrose (6 mg/kg/min) and bicarbonate (1 mEq/kg/hr) were continuously infused. Hemodynamic parameters during LV ranged from: heart rate = 146-202 b/min; mean arterial pressure = 40-80 mmHg; central venous pressure = 0.7-9.8 mm/Hg; O2 consumption = 1.38-11.8 ml/min/kg. Stable gas exchange was demonstrated with; PaO2 = 158-222 mmHg; PaCO2 = 34-38 mmHg; and pH = 7.28-7.39. The extrauterine model provides general accessibility of the preterm animal thus enabling the necessary instrumentation for comprehensive evaluation. Of further importance, the viability of preterm animals is extended to an earlier gestational age, thereby enlarging the scope of developmental research. (Supported by NIH Grant HL22843 and 5T32HL0741405)
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Bhutani, V., Wolfson, M., Tran, N. et al. AN EXPERIMENTAL PRETERM MODEL OF EXTRAUTERINE DEVELOPMENT. Pediatr Res 18 (Suppl 4), 312 (1984). https://doi.org/10.1203/00006450-198404001-01316
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DOI: https://doi.org/10.1203/00006450-198404001-01316