Abstract
We examined nasal secretions (NS) of 13 patients (pts) age 1-8 months (mos) hospitalized with acute RSV infection for IgA Ab to the surface glycoproteins (66K and 84K) and nucleocapsid protein (NCP) of RSV. Acute and convalescent (conv) NS (obtained 3-17 days (d) after initial NS) were examined by enzyme-linked immunosorbent assay (ELISA) using individual mouse monoclonal Abs (RSV Long strain) to 66K, 84K, and NCP and RSV hyperimmune horse serum (HARS) as capture Abs. Competition assays demonstrated that the antigens captured in this system were specific for each monoclonal Ab used. Total IgA Ab to RSV measured by HARS showed a ≥ 3-fold rise in Ab titer in 9/13 pts. Ab rise (≥ 3-fold) to 66K (fusion) glycoprotein occurred in 5/13 pts; 84K, 3/13 pts; and NCP, 6/13 pts. Ab rise was most striking in the 7 pts with later conv NS (≥ 6 d after acute NS). All but the youngest (1 mo) of these 7 pts had a ≥ 3-fold rise in total RSV Ab measured by HARS capture. Three, 2, and 4 of these pts had Ab rises to the 66K, 84K, and NCP respectively. Only 2/13 pts, both aged 1 mo, had no detectable RSV-specific Ab in NS. Ab rise in the remaining pts could not be explained by differences in IgA content between acute and conv NS. We conclude that monoclonal Ab capture ELISA can be used to detect class-specific Ab in infant NS and that infants recovering from RSV make IgA Ab to NCP and both surface glycoproteins.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burns, J., Hendry, R., Ho, J. et al. ANTIGEN-SPECIFIC SECRETORY ANTIBODY (Ab) RESPONSE FOLLOWING RESPIRATORY SYNCYTIAL VIRUS (RSV) INFECTION IN INFANTS. Pediatr Res 18 (Suppl 4), 270 (1984). https://doi.org/10.1203/00006450-198404001-01065
Issue Date:
DOI: https://doi.org/10.1203/00006450-198404001-01065