Abstract
Serum asialoglycoproteins bind to the hepatocyte receptor HBP, are internalized and catabolized in lysosomes. Human fetal liver is said to lack receptor, while adult liver contains 10 ug/gm determined as cpm I125-asialoorosomucoid(ASOR) substrate bound.
Female CD-1 mice produced eight timed litters of 10-14 pups, which were killed between birth and 23 days of age. Seven females were killed during pregnancy, immediately after delivery and during suckling. Virgin female and male mice were studied at 6 weeks and 4 months of age. After cervical dislocation, liver was removed, frozen at-70°C and assayed within 30 days. Aliquots of liver were homogenized, incubated with I125-ASOR (prepared from pooled human sera) in 0.1M Tris pH7.9 containing 0.1% Triton X-100 at 30° for 10 minutes. HBP-I125 ASOR complex was precipitated with 10% polyethyleneglycol, filtered, washed and precipitate cpm determined. Binding could be inhibited by specific anti-rat HBP antibody.
Fetal HBP activity was detectable one day prior to birth and rose to a plateau value at 5 days of age with no further increase to 6 weeks. In the last five days of pregnancy, maternal liver HBP activity was increased 150%. These data suggest fetal liver HBP is not involved in fetal glycoprotein metabolism, but HBP is inducible in maternal liver during pregnancy and in pups in the perinatal period. (NIAMDD Grant AM 17702).
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Collins, J., Stockert, R., Morell, A. et al. 534 HEPATIC BINDING PROTEIN (HBP)-ONTOGENY OF THE ASIALOGLYCOPROTEIN RECEPTOR IN MICE. Pediatr Res 15 (Suppl 4), 529 (1981). https://doi.org/10.1203/00006450-198104001-00547
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DOI: https://doi.org/10.1203/00006450-198104001-00547