Abstract
Alpha adrenoceptors(AAC), beta adrenoceptors(BAC) and dopamine receptors(DR) were characterized in isolated rat G & T. Specific binding(SB) was the difference between binding of the ligand alone and in the presence of an excess (100 μM) of a specific competitor. The pairs used were 3H-WB4101 and l-norepinephrine (AAC), 3H-dihydroalprenolol and l-propranolol(BAC) and 3H-haloperidol and l-flupenthixol(DR). SB was rapid, reversible, linear to protein concentration and stereospecific. The potency of agonists and antagonists in competition studies was consistent for the specific receptor being studied. Apparent dissociation constant (Kd) and receptor occupancy (Ro) were calculated from Scatchard plots. The results (mean±SEM) are tabulated:
For AAC, Ro and Kd were similar in G and T. For BAR, Ro was higher in G than in T but Kds were similar. For DR, Ro & Kd were higher in G than in T. These studies give the first evidence for specific DR in rat kidney. The presence of specific catecholamine receptors in G may explain the ability of adrenergic and dopaminergic agents to influence glomerular ultrafiltration.
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Felder, R., Pelayo, J., Blecher, M. et al. 404 ADRENERGIC AND DOPAMINERGIC RECEPTORS IN GLOMERULI(G) AND CORTICAL TUBULES (T). Pediatr Res 15 (Suppl 4), 507 (1981). https://doi.org/10.1203/00006450-198104001-00415
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DOI: https://doi.org/10.1203/00006450-198104001-00415