Abstract
The Raji cell assay (RA) and Clq binding test (ClqBT) were used to monitor changes in IC levels over 1 to 15 mos. in 17 patients with NB, 3 of whom were stage IVs. During the period of observation 19 tumor recurrences and 7 deaths were observed. IC were found in 10 of 62 samples (16.1%) by ClqBT and in 29 of 62 samples (46%) by RA. Changes in serum ClqBT or RA did not correlate with changes in disease activity or tumor burden. Sera from NB patients but not from age-matched controls precipitated a protein in 125I extrinsically labeled extracts of the LA-N1 NB cell line which had the molecular weight and antigenic characteristics of BSA. Using a modified Farr assay, 11 of 17 patients had anti-BSA antibodies; 23% of the initial samples and 14% of the subsequent 61 sera contained more anti-BSA activity than was found in 95% of sera from 13 age-matched controls. Incubation at pH 3.0 overnight in the presence of 125I-BSA, followed by neutralization, substantially increased the anti-BSA activity of 3 sera with high levels of IC but did not influence the anti-BSA activity of 3 sera with moderate to low IC. This demonstration of abnormally increased anti-BSA activity as well as “blocked” anti-BSA activity in IC suggests an altered reactivity to milk antigens or abnormal regulatory control of humoral immune responses in NB.
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Beck, D., Rossen, R. & vanEys, J. 787 ANTI-BOVINE SERUM ALBUMIN (BSA) ANTIBODIES ARE A COMPONENT OF CIRCULATING IMMUNE COMPLEXES (IC) IN CHILDREN WITH NEUROBLASTOMA (NB). Pediatr Res 15 (Suppl 4), 573 (1981). https://doi.org/10.1203/00006450-198104001-00811
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DOI: https://doi.org/10.1203/00006450-198104001-00811