MICROMETHOD FOR DETERMINATION OF PLASMA ZINC IN PRETERM INFANTS

Article metrics

Abstract

Atomic absorption spectrophotometry (A.A.S.) flame techniques use 0·2-0·5 ml plasma. A.A.S. with Electrothermal atomisation is more sensitive and requires <20 μ1 of plasma, but contamination is a problem and interference from the plasma matrix prevents the use of aqueous standards. Therefore the zinc in sheep serum was removed with Chelex 100 and the residual zinc(<0·05 μg/m1) analysed by flame A.A.S. Zinc standards ranging from 0·1 to 2·0 μg/m1.Zn were prepared in this serum. Only acid washed glass, polypropylene or P.T.F.E. equipment was used. A single 50 μ1 Oxford pipette tip, once cleaned, was used to check the distilled water (<0·2ng/ml.Zn) and then for the whole days work, being rinsed in 5% Aristar HNO3×10 and H2×10 between injections. Standards and samples were diluted 1:200 by a Micromedic diluter into polypropylene tubes and 50 μ1 injections made into the furnace. Precision studies give a Coefficient of variation of 2·5%. Recovery experiments gave results 98 ± 2% of expected value with 95 ± 5% recovery of the amount added.

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shaw, J., Bury, A., Barber, A. et al. MICROMETHOD FOR DETERMINATION OF PLASMA ZINC IN PRETERM INFANTS. Pediatr Res 14, 1420 (1980) doi:10.1203/00006450-198012000-00070

Download citation