Abstract
The G.c. properties have been studied in isolated glomeruli from normal and PAN rats (after a single I.V. injection of puromycine of aminosid).
In normal rats, the G.c. activity is elevenfold lower in mechanical disrupted glomeruli than in intact glomeruli. The effects of agents, known for their stimulation effect on soluble enzyme (NaN3) or membrane bound enzyme (Triton), suggest that the glomeruli G.c. is essentially membrane bound. After fractionnation and 105 000 g centrifugation the data show the liberation of the membrane bound G.c. and probably the existence of a membrane bound effector able to inhibit this enzyme.
12 days after PA injection, the G.c. activity in intact glomeruli is not statistically different to the normal its. But in disrupted glomeruli (homogenate) the G.c. activity is up to 300% higher than in normal rats. The differences seen after fractionnation and 105 000 g centrifugation between normal and PAN rats suggest that the G.c. activity is more stable in PAN glomeruli, demonstrated furthermore by progressive ultrasonication. The G.c. properties and stability changes could be attributed to a membrane structure change of the 12 days-PAN glomerular cells. Therefore the G.c. activity regulation is involved in the glomerular functions changes occuring in the nephrotic rats.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Geisert, J., Helwis, J., Yusufi, A. et al. Properties of the guanylate cyolase (G.c.) activity lnn in puromycine of aminosid nephrotic (PAN) rats. Pediatr Res 14, 1004 (1980). https://doi.org/10.1203/00006450-198008000-00186
Issue Date:
DOI: https://doi.org/10.1203/00006450-198008000-00186