Abstract
Acetone-induced precipitation of the 1:1 serum albumin-bilirubin complex, followed by treatment with 7M guanidine, allows complete removal of bilirubin from albumin by gel filtration. After illumination in the 440-470nm wavelength region (300 μW/sq.cm/nm), bilirubin or its photoproduct(s) cannot be removed any more from albumin. This was also confirmed by gel filtration on Sephadex G-75 (30% acetic acid as eluant) of BrCN-treated albumin, after 30 min. irradiation. This suggests the formation of a covalent binding which was detected also "in vivo" in the sera of jaundiced newborns. After 7 hrs.phototherapy with 4 F2OT12/BB Westinghouse lamps (irradiation intensity at the infant level = 22 μW/sq.cm/nm, in the range of 440-470 nm), the covalent adduct began to appear. The quantity of the adduct was dependent upon the amount of light energy received during treatment. The adduct disappeared at 15 to 20 days after treatment.
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Rubaltelli, F., Rossi, E. & Jori, G. Evidence for visible light-induced covalent binding between bilirubin and serum albumin “in vitro” and “in vivo”: 66. Pediatr Res 14, 176 (1980). https://doi.org/10.1203/00006450-198002000-00093
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DOI: https://doi.org/10.1203/00006450-198002000-00093