Abstract
Congenital athymic nude /nu/nu/,heterozygous /nu+/ and BloA 6 weeks old mice were used for studies on their response to L-Norepinephrine.In twelve experiments cell suspension of lymphnodes were prepared and the cells were incubated in culture medium containing 1.o or o.1 mM L-Norepinephrine./2-83H/-adenine was used and net cyclic AMP production was measured after deproteinisation by thin-layer chromatography.Lymphocyte basal cyclic AMP content /expressed in pM cyclic AMP/1o7 cells/ was 4.0±0.53 in the nu/nu group,17.75± 9.6 in the nu/+ lymphocytes and 11.12±1.3 in B1oA animals.Incubation for four minutes with 1.o mM L-Norepinephrine produced an increase of 218 per cent /38.75±9.5/ in nu/+ and 586 per cent /65.25±12 / in BloA lymphocytes.o.1 mM L-Norepinephrine resulted in 3o2 per cent increase of cyclic AMP content /53.66±16/ in nu/+ and 971 per cent /109.0±25/ in BloA animals.In contrast,athymic nude lymphocytes did not show any significant cyclic AMP content change in response to L-Norepinephrine at any concentrations.The results suggest,that primarily T cells are involved in the cyclic AMP response induced by L-Norepinephrine.
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Cser, á., Fachet, J. & Hules, H. Cyclic AMP production in mouse lymphocytes in response to L-Norepinephrine. Pediatr Res 13, 84 (1979). https://doi.org/10.1203/00006450-197901000-00089
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DOI: https://doi.org/10.1203/00006450-197901000-00089