Abstract
Normal T cells and null cells produced an inhibitor (SIF) of both phytohemagglutinin (PHA) induced T cell transformation (15,793 CPM of 3H-thymidine incorporation reduced to 2,731 CPM - 83% inhibition) and formation of sheep red blood cell plaques (645 plaques/106 cells reduced to 219 plaques/106 cells) by stimulated B cells. T cells also produce a factor (LMF) which induces B cells to proliferate and synthesize immunoglobulin. Stimulated T cells produced SIF continuously by 5 days of culture while LMF production ceased after 72 hours. SIF and LMF were separated on Sephadex G200; SIF occurred predominantly in the exclusion column, and LMF exclusively in the postalbumin fraction. Serum from a man with an unusual skin lymphoma had potent SIF-like activity. That the serum SIF activity originated from the lymphoma was suggested by the fact that supernatants from skin tumor cells potently inhibited PHA-induced mitosis of normal T cells. The patient's peripheral blood lymphocytes were unresponsive to antigens and mitogens in autologous serum, but responded normally in homologous serum. This tumor appears to represent an abnormal extension of a physiological process.
Supported by U.S. Public Health Service Research Grant AI-05877.
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Wolf, R., Rosen, F. & Merler, E. 747 A NATURALLY OCCURRING INHIBITOR OF LYMPHOCYTE FUNCTION FROM A CUTANEOUS LYMPHOMA. Pediatr Res 12 (Suppl 4), 488 (1978). https://doi.org/10.1203/00006450-197804001-00752
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DOI: https://doi.org/10.1203/00006450-197804001-00752