Abstract
PMNS chemotactic indices (CI) and random migration (RM) were determined for 12 asymptomatic asthmatic children (mean age 7.5 years) who were receiving no medication and for an equal number of age-matched controls by a modified Boyden's technique, in which PMNs, deposited on a 3μmicropore filter, were placed in the upper chamber and endotoxin-activated AB serum (EAS) or endotoxin-activated patients' serum (EPS) in the lower chamber. For RM determinations, EAS was replaced by the Hank's balanced salt solution (HBS). EPS was also tested against patients' cells (PC) and control cells (CC). Mean (±1SD) CIs and RMs are presented in the table. Patients' CIs and RMs were lower than those of controls (P<0.002). EPS generated more chemotaction for CC (P< 0.01) than did EAS. In addition, serum IgE levels were determined on 6 of the asthmatic patients selected at random. The mean value of 733 units (range 473-1232) was about 2 1/2 times the normal value described for this age group. Defective CIs and RMs may be at least partly responsible for increased susceptibility to infection in asthmatics. Increased chemotaction by EPS may be due to unknown cytotactic factor (s). The mechanism of the PMN defects is unclear and may be related to increased IgE levels and/or deactivation of receptors by pre-existing cytotactic factor (s).
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Khan, A., Evans, H., Shin, Y. et al. 707 ABNORMAL NEUTROPHIL (PMNS) CHEMOTAXIS AND ELEVATED IgE IN CHILDHOOD ASTHMA. Pediatr Res 12 (Suppl 4), 481 (1978). https://doi.org/10.1203/00006450-197804001-00712
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DOI: https://doi.org/10.1203/00006450-197804001-00712