Abstract
The bactericidal defect of CGD PMNs has been partially corrected in previous studies relying on the co-phagocytosis of bacteria and of either glucose oxidase (GO)-coated latex particles or GO-containing liposomes. We investigated the effect of opsonization of bacteria with an IgG-GO conjugate upon the bactericidal activity of CGD PMNs and monocytes (MC). Anti-staphylococcal rabbit IgG was prepared and conjugated to GO by diethylmalonimidation. The conjugate (final concentration of IgG: 2.0 mg/ml) was used to opsonize bacteria which were then ingested by PMNs or MCs of female CGD carriers. In control preparations IgG alone was used as opsonin. After 120 minutes of incubation with CGD PMNs, the number of surviving intracellular bacteria decreased from a mean of 42.3% (opsonin: IgG) to 17.7% (opsonin: IgG-GO conjugate). In MCs of CGD carriers the number of intracellular bacteria decreased by 45%. In PMN-free preparation the IgG-GO conjugate displayed negligible bactericidal activity. The bactericidal defect of CGD phagocytic cells can be successfully corrected by the intracellular introduction of an IgG-GO conjugate. Furthermore, such restoration of bactericidal activity may be enhanced by the close proximity between the bacteria and the hydrogen peroxide-generating opsonin.
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Humbert, J., Weston, W. & Dearmey, P. 704 CORRECTION OF NEUTROPHIL (PMN) DYSFUNCTION IN CHRONIC GRANULOMATOUS DISEASE (CGD) WITH AN IgG-OXIDASE CONJUGATE. Pediatr Res 12 (Suppl 4), 481 (1978). https://doi.org/10.1203/00006450-197804001-00709
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DOI: https://doi.org/10.1203/00006450-197804001-00709