Abstract
Decreased survival has been noted for RBC of newborn man and rat(Ped.Res.11:1155,1977).The present studies investigate the possibility that decreased survival is due to an altered RBC membrane. Osmotic fragility of newborn(NB) rat RBC was increased (29% lysis in 0.5% NaCl;adult RBC:6%),increasing to 92% following 1 hr incubation at 20° in pH 9.2 borate buffer (adult RBC: no change). Filtration half-time of RBC suspensions was prolonged (NB:40 min; adult:2-3 min)and returned to normal with advancing age(16 min at 6 days,5 min at 12 days,3 min at 26 days). Splenic uptake at 24 hr was increased 15-fold & hepatic uptake increased 3-fold for 51Cr-labeled NB RBC as compared to adult RBC. Following treatment with the cross-linking agent dimethyladipimidate (DMA,2-5mM,pH 9.2),survival of 51Cr-labeled adult RBC was slightly reduced from 100&92% at 1&24 hr to 94&76%. DMA(2-5mM) caused marked further reduction in survival of NB RBC from 64&16% at 1&24 hr to 10&3%. Following incubation of intact RBC with glucose, electrophoresis of RBC membrane proteins yielded a pattern of high molecular weight complexes(HMWC) for NB,but not for adult, RBC. These results suggest an unstable RBC membrane in the newborn rat,leading to altered osmotic fragility,reduced deformability, splenic trapping, and reduced survival. Preferential hemolysis of DMA-treated NB rat RBC and HMWC formation suggest that an altered state of aggregation of membrane proteins may underlie these abnormalities.
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Landaw, S., Oski, F. 192 DECREASED SURVIVAL AND ALTERED MEMBRANE PROPERTIES OF RED BLOOD CELLS (RBC) IN THE NEWBORN RAT. Pediatr Res 12 (Suppl 4), 395 (1978). https://doi.org/10.1203/00006450-197804001-00197
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DOI: https://doi.org/10.1203/00006450-197804001-00197