Abstract
Prostaglandin (PG) biosynthesis by the mammalian kidney occurs mainly in the medulla (M) and PG breakdown takes place mainly in the cortex (C). We have studied the PG synthetase system in 6 human newborn kidneys (16,22,26,28,31,32 weeks of gestation) 2 hours after death. Slices of M and C were incubated in a medium containing 50 μg arachidonic acid (AA) and 50 μCi [3H]-AA. The incorporation of [3H]-AA into PGE, PGF and PGA was determined at 30-min. intervals in M and C tissue and in the incubation medium over 3 hrs. using silicic acid chromatography and scintillation counting. [3H]-PG's were not detected in C tissue but PGA was detected in C medium in infants ≥26 weeks of gestation. After 1 hr., PGs (mean values) in the M medium were (cpm/mg tissue):
The renal concentration of the essential fatty acids which are PG precursors varied only slightly with advanced gestation. Detection of different PG compounds may relate to changes occurring in the PG synthetase system complex, in PG metabolism or both during maturation. The study demonstrates that the renal medulla and cortex are associated with PC synthesis in infants ≥26 weeks of gestation and that the renal medulla is the main site for PG synthesis in the maturing kidney ≥28 weeks of gestation.
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Friedman, Z., Demers, L. & Nelson, N. 183 ROSTAGLANDIN SYNTHETASE IN THE HUMAN NEONATAL KIDNEY. Pediatr Res 12 (Suppl 4), 394 (1978). https://doi.org/10.1203/00006450-197804001-00188
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DOI: https://doi.org/10.1203/00006450-197804001-00188