Abstract
Addition of serum to 3T3 cells arrested in Go induces the cells to enter and progress through G1; DNA synthesis follows after a 12-14 hour lag period. Whole serum has been fractionated into two sets of components which act synergistically to stimulate the replication of 3T3 cells. One is found in heat(100oC) treated platlet extracts(PE), and the other is found in platlet-poor serum(PPS). We have examined the relation of RNA synthesis to subsequent DNA synthesis using whole serum and these two components. RNA in 3T3 cells was labeled to constant specific activity with 32PO4. We found that whole serum or its components (PE and PPS) stimulated an increase in RNA content to the same degree in quiescent cultures of 3T3 cells. Each agent increased messenger RNA(mRNA) content more than ribosomal RNA(rRNA) content and the changes in mRNA occurred before detectable changes in rRNA. Stimulation with either whole serum or PE induced both RNA and DNA synthesis; however, PPS induced only RNA synthesis. Cells stimulated by PPS remain in Go since the subsequent addition of PE does not shorten the latent period for DNA synthesis. Therefore, increased RNA synthesis and content does not always lead to DNA synthesis and cell division; PPS mediates this decoupling. Increased RNA synthesis, therefore, cannot itself be considered a criterion for entry of G0 cells into G1.
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Abelson, H., Antoniades, H. & Scher, C. 170 DE-COUPLING OF RNA AND DNA SYNTHESIS IN PLATLET-POOR SERUM STIMULATED Go ARRESTED BALB/C 3T3 FIBROBLASTS. Pediatr Res 12 (Suppl 4), 392 (1978). https://doi.org/10.1203/00006450-197804001-00175
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DOI: https://doi.org/10.1203/00006450-197804001-00175