Abstract
Summary: Guanylate cyclase, cGMP phosphodiesterase and protein kinase activities were determined in kidneys of developing and adult rats. Guanylate cyclase activities of crude kidney homogenates, 100,000 × g supernatant and pellet of 7− and 21-day-old and adult rats were determined (Table I). In the kidneys of 7-day-old rats activity was 162% of adult controls in the homogenates (P < .001), 144% in the soluble (P < .005) and 308% in the particulate fraction (P < .001). In 3-week-old rats activity was still significantly higher at 144% in the homogenate (P < 0.02) and at 225% in the particulate (P < .001). Phosphodiesterase activity for cGMP was 7488 ± 831 pmol cGMP/mg protein · min in 1-week-old and 7674 ± 1120 in 3-week-old rats vs. 4042 ± 122 in the adults (P < .025) (Table II).
Chromatography on Sephadex G-200 showed two peaks of cGMP-stimulatable protein kinase in both the adult and newborn kidney and in addition a minor peak of cGMP-stimulatable kinase in the newborn kidney only (Fig. 2).
Speculation: These results provide further evidence for altered cGMP metabolism during kidney development. Whether cGMP is directly involved in organ growth remains open to speculation.
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Schlondorff, D., Trizna, W. Increases of Guanosine 3′,5′-Monophosphate-related Enzymes in Kidneys of Developing Rats. Pediatr Res 12, 882–885 (1978). https://doi.org/10.1203/00006450-197808000-00017
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DOI: https://doi.org/10.1203/00006450-197808000-00017