Abstract
Activities of superoxide dismutase (SOD), catalase (CATA), and glutathione peroxidase (GPX) of AM from guinea pigs (GP) exposed continuously to 50 or 85% oxygen have shown divergent patterns. At 50% oxygen both SOD and GPX activities increased 2-fold by 18 hours while CATA remained the same. After 18 hours of exposure to 85% oxygen, SOD, GPX, and CATA activities were similar to control. This study evaluated the effect of increasing oxygen tensions on these enzymes to explain the different patterns. GP were exposed to oxygen tensions between 30% and 85% for 18 hours and AM were harvested and purified on a Ficoll-hypaque gradient. SOD activity in room air was 3.4 ± .3 (x ± SD) units/mg protein and progressively increased to peak levels of 6.0 ± .4 at 50%, and subsequently decreased to control levels at 85% oxygen. GPX activities increased from 10.4 ± 2.7 for controls to peak at 21.7 ± 6.6 units/mg protein at 60%, and decreased to control levels at 85% oxygen. Control CATA activity was 355 ± 34 units/mg protein and exhibited no significant changes. Monocyte non-specific esterase in purified AM showed no differences between room air controls and 50% oxygen but were decreased at 85% oxygen. These studies show that SOD and GPX vary in relationship to oxygen tensions and may be the result of enzyme activation and/or induction, or heterogeneity of the AM population.
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Johnson, G., Baehner, R., Higgins, C. et al. 1196 THE EFFECT OF VARYING OXYGEN TENSIONS ON SUPEROXIDE DISMUTASE, CATALASE, AND GLUTATHIONE PEROXIDASE ACTIVITIES IN ALVEOLAR MACROPHAGES (AM) FROM GUINEA PIGS. Pediatr Res 12 (Suppl 4), 563 (1978). https://doi.org/10.1203/00006450-197804001-01202
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DOI: https://doi.org/10.1203/00006450-197804001-01202