Abstract
We have demonstrated that DPL is the most effective phospholipid in establishing alveolar stability when used in combination with unsaturated PG. The purpose of this study was to determine the ability of different molar ratios of DPL/u-PG to restore the lung PV characteristics to normal. The surfactant (S) depleted adult rat lung model was used. For all PV curves the % of original total lung capacity (%TLC) at 5 cm H2O on the deflation limb was calculated. PV measurements were made three times on each lung: prior to depletion of S (mean 54.9%), after maximal removal of S by lung washing (mean 10.0%), and after the instillation of a 2 ml DPL/u-PG suspension into the trachea. DPL/u-PG suspensions were prepared by suspending various molar ratios of DPL and u-PG in saline and sonicated at 0°C. The concentration was 5 mg of Phospholipid/ml. The results for each of the different ratios of DPL/u-PG suspension were: 10/0, 26%; 9/1, 37%; 7/3, 39%; 5/5, 40%; 4/6, 26%. Surface tension measurements were made using a dynamic alveolar model. The minimum surface tensions (γ min. dynes/cm) were as follows: 10/0, 59; 9/1, 5; 7/3, 6; 5/5, 24; 4/6, 62; 0/10, 55. These findings indicate that DPL in combination with u-PG might be a good synthetic surfactant for the treatment of Respiratory Distress Syndrome if DPL is combined with u-PG in ratios of 9/1; 7/3 and 5/5.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ikegami, M., Adams, F. 1193 RESTORATION OF LUNG PRESSURE-VOLUME (PV) CHARACTERISTICS WITH VARIOUS RATIOS OF DIPALMITOYL LECITHIN (DPL) AND PHOSPHATIDYL GLYCEROL (PG). Pediatr Res 12 (Suppl 4), 562 (1978). https://doi.org/10.1203/00006450-197804001-01199
Issue Date:
DOI: https://doi.org/10.1203/00006450-197804001-01199