Abstract
A prospective study was undertaken to determine if urinary LDH isoenzyme assays could differentiate site of infection in patients with urinary tract infection. Thirty children, with a mean age of 6.1 years (23 female and 7 male), were assigned to control, cystitis or pyelonephritis categories based on clinical, laboratory and radiologic criteria. LDH isoenzyme concentrations were measured by a thin film agarose electrophoresis (Pol-E-Stret, Pfizer Co.) and expressed as % of I, II, III, IV and V. Of the 10 controls, one had isoenzyme IV and V in 1.2% and 2.3% concentrations. Of 11 patients with cystitis, one had isoenzyme IV and V in 2.6% and 1.3% concentrations. However, in all 9 patients with pyelonephritis, there was a significant percentage concentration of LDH IV (13.8 ± 8.08) and V (15.1 ± 9.8) activity in the urine. It was concluded that patients with pyelonephritis had significant concentrations of LDH IV and V iso-enzymes in their urine, unlike normal children or those with cystitis. The source of these enzymes is postulated to be the renal parenchyma itself as modified by the disease process. The diagnosis of site of infection could be made by measuring LDH isoenzyme IV and V concentration in all the patients with urinary tract infection.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Devaskar, U., Montgomery, W. & Hodgman, J. 1059 URINARY LACTIC DEHYDROGENASE (LDH) ISOENZYME IV AND V IN THE DIFFERENTIAL DIAGNOSIS OF PYELONEPHRITIS AND CYSTITIS. Pediatr Res 12 (Suppl 4), 540 (1978). https://doi.org/10.1203/00006450-197804001-01065
Issue Date:
DOI: https://doi.org/10.1203/00006450-197804001-01065