Abstract
Defective masculinization may be due to absent binding of androgen to the target organ receptor. Cytosol prepared directly from a fragment of foreskin was incubated with 3H-DHT and analysed by polyacrylamide gel electrophoresis. 10 prepubertal subjects, 5 adults, 4 boys with anterior hypospadias and 3 MPH were studied. Two binding peaks were seen. One was displaced by cyproterone acetate and methyltrienolone but not by estradiol, was modified by high ionic strength and corresponded to the intracellular receptor. The other was the plasma sex binding protein. Receptor binding was similar in newborns, normal boys and anterior hypospadias (≃30 f.moles/mg cell prot.). Higher values were found in adults (≃80 f.moles). No receptor was found in familial MPH Type I, and incomplete testicular feminization but was present in mixed gonadal dysgenesis. This method permits a rapid assessment of androgen receptivity.
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Evain, D., Savage, M., Binet, E. et al. Dihydrotestosterone (DHT) receptor in human foreskin cytosol. Studies in normal subjects and male pseudohermaphrodites (MPH). Pediatr Res 12, 152 (1978). https://doi.org/10.1203/00006450-197802000-00035
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DOI: https://doi.org/10.1203/00006450-197802000-00035