Abstract
Bioactive factors affecting ciliary activity, leucocyte degranulation and Na+ resorption appear to accumulate in fluids of cystic fibrosis (CF) patients. We have investigated the presence of abnormal amounts or species of polypeptides (m.w. ≥ 20,000 daltons) in serum and plasma of 10 CF patients as compared to 10 age, sex matched normals and contrast patients. Freshly prepared citrated plasma or serum was fractionated by column chromatography on a Biogel P10 column (2.5×53cm) or Sephadex G50 column (2.5×100cm), eluted respectively with 0.1 M NH4HC03 (pH8.3) or 0.01 M P04 buffer (pH7.4), and the elution profile monitored at 220nm. Six distinct peaks (m.w. 2-10,000) occurred in remarkably constant proportions in both CF and non-CF plasma specimens, while serum from each contained only 5 peaks. No consistent differences in amounts of these species were detected in cystics nor were any new peaks present. Further fractionation of major peaks on DEAE-Sephadex A25 gave no evidence of differences in the plasma peptide profile of cystics vs. normals. Based upon peptide standards of known m.w., the sensitivity of this spectrophotometric method is in the range of 0.5-5 nmole/ml blood. Therefore, if novel polypeptide factors do exist in CF, their concentration is below this level.
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Rennert, O., Seale, T., Chan, WY. et al. 886 NO DIFFERENCES DETECTED SPECTROPHOTOMETRICALLY BETWEEN BLOOD PEPTIDES OF CYSTIC FIBROSIS AND NORMAL INDIVIDUALS FRACTIONATED BY COLUMN CHROMATOGRAPHY. Pediatr Res 12 (Suppl 4), 511 (1978). https://doi.org/10.1203/00006450-197804001-00891
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DOI: https://doi.org/10.1203/00006450-197804001-00891