Abstract
Fructose-1,6-diphosphatase (FDPase) is a liver gluconeogenic enzyme. FDPase deficiency has been described which is characterized by severe hypoglycemic episodes, metabolic acidosis, and hepatomegaly. FDPase deficiency may be diagnosed by the enzyme assay only if liver tissue is utilized. For the study of FDPase activity in tissues other than liver, human term placentas were used. Placental slices were washed with cold 0.15 M NaCl then sonicated in 0.2 M glycine buffer, pH 9.2, containing 0.1 M MnCl2, then centrifuged at 10,000 xg for 10 min. The supernatant fractions were assayed for FDPase activity using fructose-1,6-diphosphate as substrate. The hydrolyzed phosphate was estimated by the method of Fiske and SubbaRow. Extracts from normal human placentas hydrolze 1.5 uM phosphate/mg protein/hr. In order to ascertain that the phosphate hydrolyzed is from Fructose-1,6-diphosphate, a labeled substrate U-(14C)-Fructose-1,6-diphosphate was used and the hydrolysis products were determined by thin layer chromotography using 80% propanol as solvent. Full term placental extracts hydrolyze 40% of the radioactive substrate per 100 ug of protein per hour. These data indicate the presence of FDPase in placental tissues which may prove useful in the study of pregnancies at risk for FDPase deficiency. This assay may be used for the study of the gluconeogenesis in human placenta. Supported by Nat'l Foundation Grants - 244-39-66-321 and 244-39-66-317
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matalon, R., Justice, P., Michals, K. et al. 879 FRUCTOSE-1.6-DIPHOSPHATASE ACTIVITY IN HUMAN TERM PLACENTA. Pediatr Res 12 (Suppl 4), 510 (1978). https://doi.org/10.1203/00006450-197804001-00884
Issue Date:
DOI: https://doi.org/10.1203/00006450-197804001-00884
This article is cited by
-
Gluconeogenic enzymes in the human placenta
Journal of Inherited Metabolic Disease (1984)