Abstract
In vitro semisolid agar culture has been used to characterize and predict relapse in myelold leukemias. We evaluated 5 children with ALL on the same drug regimen with sequential BM and CFC on day 0, 14, 28, and 83 or 138 to characterize the CFC in ALL and compare cytologic methods to CFC to predict chemotherapeutic effect and relapse. Feeder underlayers were prepared from 1×106 normal human WBC in 0.3% agar and McCoys media as a source of CSF; 2×106 nucleated marrow cells were overlayered. Colonies were counted at day 14 and compared to the peripheral blood absolute neutrophll count (ANC) and Giemsa stained BM. BM cellularity was ranked from 0–4 (3=normocellular).
There was poor correlation between CFU-C and ANC. However, an increase in CFU-C was noted with impending remission induction as the BM was reconstituted with myelold precursors. This was most consistently noted when serial determinations were performed on an individual. In one patient no increase in CFU-C was noted despite cytologic evidence of remission; this patient subsequently relapsed. Serial assessment of CFU-C may be helpful in predicting relapse before morphological evidence on routine BM preparations, but does not help in predicting chemotherapeutically induced neutropenia.
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Luban, N., Miller, D. SERIAL ASSESSMENT OF BONE MARROW (BM) COLONY FORMING CAPACITY (CFC) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA (ALL). Pediatr Res 11, 475 (1977). https://doi.org/10.1203/00006450-197704000-00632
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DOI: https://doi.org/10.1203/00006450-197704000-00632