Abstract
PB from a 5 y.o. child with untreated adult-type (Ph1 chromosome positive) CGL was studied for in-vitro leukocyte colony production. Both the number of colonies obtained and the morphologic nature of the cells in the colonies were assessed. Unfractionated buffy coat produced 21 ± 5 colonies/105 cells. When buffy coat was fractionated by centrifugation over ficoll-Hypaque, colony forming units (CFU-c) were found to be concentrated in the low density top layer containing myeloblasts, promyelocytes, and myelocytes (70 ± 5 colonies/105 cells were obtained from this layer). The bottom layers contained metamyelocytes, bands, and polymorphonuclear cells, but no CFU-c. When studied morphologically and histochemically the vast majority of colonies derived from both whole buffy coat and from the low density cell layer were found to be comprised of mature granulocytes, a result consistent with that described for a-dults with CGL. We have previously reported that colonies formed from the PB of children with so called juvenile-type (Ph1 chromosome negative) CGL are exclusively monocytic in nature. It would thus appear that in children with Ph1 chromosome positive CGL (like adults with the same condition), the leukemic process affects predominantly the granulocytic precursor cells whereas in children with Ph1 chromosome negative CGL, the monocyte precursor is the cell most prominently involved.
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Altman, A., Baehner, R. PRODUCTION OF GRANULOCYTIC COLONIES IN-VITRO FROM THE PERIPHERAL BLOOD (PB) OF A CHILD WITH ADULT-TYPE CHRONIC GRANULOCYTIC LEUKEMIA (CGL). Pediatr Res 8, 397 (1974). https://doi.org/10.1203/00006450-197404000-00340
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DOI: https://doi.org/10.1203/00006450-197404000-00340