Abstract
A relationship between steroidogenesis and RNA metabolism has been suggested by the increased synthesis of rapidly labelled adrenal RNA at the time of the initiation of the steroidogenic response to ACTH (Endocr. 93:285,73; Steroids 22: 171,73). To further determine the role that RNA synthesis may play during ACTH-induced steroidogenesis, actinomycin D, an inhibitor of DNA-directed RNA synthesis was injected into rats at 10μg/100g body weight. The rats were sacrificed at 24 hrs. later. 25mC of uridine 5-6 H3 and 3 USP units of ACTH were injected 2 hrs. before death. The control group did not receive actinomycin. The similar increase in plasma corticosterone levels in actinomycin-treated rats and controls indicated that this inhibitor had no effect on the steroidogenic action of ACTH. There were no differences in the incorporation of labelled precursors into the total cytoplasmic RNA and into the different fractions of RNA separated by gel electrophoresis. It appears that early effects of ACTH on steroidogenesis and RNA metabolism are not related to DNA-dependent RNA synthesis.
Supported by National Sciences Foundation grant No. GB-16614
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Castells, S., Addo, N. & Kwateng, K. EFFECTS OF ACTINOMYCIN D ON ACTH-INDUCED STEROIDOGENESIS AND RNA SYNTHESIS. Pediatr Res 8, 367 (1974). https://doi.org/10.1203/00006450-197404000-00164
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DOI: https://doi.org/10.1203/00006450-197404000-00164