Abstract
Glomerular basement membranne (GBM) is composed of a collagen-like component and a carbothydrate rich glycoprotein. GBM fragments have been found in the urine of normal and nephritic man and experimental animals. This study was designed to isolate, purify and chemically and immunologically characterize GBM fragments of rat urine.
A glycoprotein, the major protein innormal rat urine (MUP), was isolated by DEAE ion excahange chromatography and purifed by gel filtration on Sephadex G 200. MUP was shown to be a cabohydrate rice glycoprotein with an amino acid content similar to the non-collagen component of GBM. Fluorescein conjugated antisera could be absorbed with rat GBM, rat glomeruli, 8 M urea solubilized GBM but not by rat collagen or the collagen component of GBM. The glycoprotein is similar to albumin in molecular size. In experimental nephrosis, the excretion of MUP is increased and the content of hexose altered.
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Wong, S., Kulvinskas, C., Kaufman, D. et al. Chemical and Immunologic Characteristics of Glomerular Basement Membrane Fragments in Rat Urine. Pediatr Res 4, 448 (1970). https://doi.org/10.1203/00006450-197009000-00058
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DOI: https://doi.org/10.1203/00006450-197009000-00058