Original Article | Published:

Functional Polymers

Cisplatin delivery vehicles based on stabilized polymeric aggregates comprising poly(acrylic acid) chains

Polymer Journal volume 49, pages 607615 (2017) | Download Citation

Abstract

Stabilized polymeric aggregates (SPAs) comprising poly(acrylic acid) (PAA) chains were studied as a delivery platform for cisplatin. SPAs were prepared by blending a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) triblock copolymer with a poly(acrylic acid)-block-poly(propylene oxide)-block-poly(acrylic acid) triblock copolymer and additional loading and photocrosslinking of pentaerythritol tetraacrylate (PETA). Dynamic light scattering analysis revealed particles with a hydrodynamic diameter of 176 nm and a monomodal particle size distribution. The stabilized polymeric aggregates were loaded with cisplatin by a ligand exchange reaction, achieving a high loading efficiency of 76%. A study on the release of complexes of platinum(II) from the particles in phosphate-buffered saline (PBS) and citrate buffer solution (CBS) at 37 °C revealed a sustained release profile. More than 90% and nearly 80% of the loaded drug were released within 312 h in PBS and CBS, respectively. The in vitro cell viability assay indicated that cisplatin immobilized in the SPAs is less cytotoxic than the non-immobilized agent. The intracellular accumulation of the entrapped complex was comparable to that of the free drug.

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Acknowledgements

We are grateful to the group of Associate Professor Radostina Alexandrova from IEMPAM-BAS for help with the cellular uptake and cytotoxicity experiments.

Author information

Affiliations

  1. Institute of Polymers, Bulgarian Academy of Sciences, Sofia, Bulgaria

    • Ekaterina Stoyanova
    • , Petar Petrov
    •  & Neli Koseva
  2. Department Analytical Chemistry, Faculty of Chemistry and Pharmacy, Sofia University ‘St Kliment Ohridski’, Sofia, Bulgaria

    • Irina Karadjova
  3. Department of Pharmacology, Pharmacotherapy, and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria

    • Georgi Momekov

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Competing interests

The authors declare no conflict of interest.

Corresponding author

Correspondence to Petar Petrov.

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DOI

https://doi.org/10.1038/pj.2017.29

Supplementary Information accompanies the paper on Polymer Journal website (http://www.nature.com/pj)