Abstract
DNA-chitosan bilayer membranes were designed and prepared as a biomedical adhesive for therapeutic application. Various DNA-chitosan bilayer membranes were prepared by applying chitosan solution (2.0 mg cm-2) on UV-irradiated DNA membranes (0.2–0.5 mg cm-2). Tensile strengths of the DNA-chitosan bilayer membranes were approximately 4.0 N similar to that of chitosan alone membrane 2.0 mg cm-2. These results indicate that the bilayer membranes have enough tensile strength as a surgical tape. When the surface of the DNA-chitosan membranes was analyzed using a scanning electron microscope (SEM), fiber-like structure was observed on the DNA-side of the DNA-chitosan membranes, prepared from more than 0.3 mg cm-2 of UV-irradiated DNA membranes. In contrast, the chitosan-side of the DNA-chitosan membranes showed a smooth surface similar to that of the chitosan alone membrane. These results indicated that a DNA-chitosan bilayer membrane was successfully prepared using UV-irradiated DNA membranes (0.3–0.5 mg cm-2) and chitosan (2.0 mg cm-2). Next, we observed bonding strength of the membranes to rabbit peritoneum. The bonding strength of the chitosan-side was similar to that of fibrin glue. On the other hand, DNA side of bilayer membrane did not adhere to the peritoneum. The DNA-chitosan membrane is bi-functional and has a potential to serve as a bi-functional bio-adhesive.
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R. A. A. Muzzarelli, Ed., “Chitin”, Pergamon Press, New York, N.Y., 1977.
S. Minami, Y. Okamoto, K. Hamada, Y. Fukumoto, and Y. Shigemasa, in “Chitin and Chitinases”, 2nd ed., P. Jolles and R. A. A. Muzzarelli, Ed., Birkhauser Verlag, Basel, 1999, p 265.
S. B. Rao and C. P. Sharma, J. Biomed. Mater. Res., 34, 21 (1997).
T. Tanigawa, Y. Tanaka, H. Sashiwa, H. Saimoto, and Y. Shigemasa, in “Advances in chitin and chitosan”, 2nd ed., C. J. Brine, P. A. Sandford, and J. P. Zikakis Ed., Elsevier Science Inc., New York, N.Y., 1992, p 206.
Y. Okamoto, M. Nose, K. Miyatake, J. Sekine, R. Oura, Y. Shigemasa, and S. Minami, Carbohydr. Polym., 44, 211 (2001).
B. C.-Gomez and R. Duncan, Int. J. Pharm., 148, 231 (1997).
S. Hirano, in “Biotechnology annual review", 2nd ed, M. R. El-Gewely, Ed., Elsevier Science Inc., New York, N.Y., 1996, vol.2, p 237.
M. Yamada, K. Kato, M. Nomizu, N. Sakairi, K. Ohkawa, H. Yamamoto, and N. Nishi, Chem. Eur. J., 8, 1407 (2002).
M. Yamada, K. Kato, M. Nomizu, K. Ohkawa, H. Yamamoto, and N. Nishi, Environ. Sci. Technol., 36, 949 (2002).
S. Matsuda, H. Iwata, N. Se, and Y. Ikada, J. Biomed. Mater. Res., 45, 20 (1999).
K. Ono, Y. Saito, H. Yura, K. Ishikawa, A. Kurita, T. Akaike, and M. Ishihara, J. Biomed. Mater. Res., 49, 289 (2000).
T. Matsumoto, R. M. Hardaway, K. C. Pani, and P. M. Margetis, Am. Surgeon., 34, 263 (1968).
Y.-C. Tseng, S.-H. Hyon, Y. Ikada, Y. Shimizu, K. Tamura, and S. Hitomi, J. Appl. Biomater., 1, 111 (1990).
H. G. Borst, A. Haverish, G. Walterbusch, and W. Maatz, J. Thorac. Cardiovasc. Surg., 84, 548 (1982).
O. Thetter, Thorac. Cardiovasc. Surgon, 29, 290 (1981).
J. A. Rousou, R. M. Engelman, and R. H. Breyer, Ann. Thorac. Surg., 38, 409 (1984).
O. J. Moy, C. A. Peimer, M. P. Koniuch, C. Howard, M. Zielezny, and P. R. Katikaneni, J. Hand. Surg., 13A, 273 (1988).
C. Remunan-Lopez, A. Portero, J. L. Vila-Jato, and M. J. Alonso, J. Controlled. Release., 55, 143 (1998).
N. Ozmeric, G. Ozcan, C. M. Haytac, E. E. Alaaddinoglu, and M. F. Sargon, J. Biomed. Mater. Res., 51, 500 (2000).
F.-L. Mi, Y.-B. Wu, S.-S. Shyu, J.-Y. Schoung, Y.-B. Huang, Y.-H. Tsai, and J.-Y. Hao, J. Biomed. Mater. Res., 59, 438 (2002).
W. Wang, S. Bo, S. Li, and W. Qin, Int. J. Biol. Macromol., 13, 281 (1991).
J. D. Smith and R. Markham, Biochem. J., 46, 509 (1950).
C.-M. Lehr, J. A. Bouwstra, E. H. Schacht, and H. E. Junginger, Int. J. Pharm., 78, 43 (1992).
P. He, S. S. Davis, and L. Illum, Int. J. Pharm., 166, 75 (1998).
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Rikimaru, S., Wakabayashi, Y., Nomizu, M. et al. DNA-Chitosan Bilayer Membrane as a Bi-functional Biomedical Adhesive. Polym J 35, 255–260 (2003). https://doi.org/10.1295/polymj.35.255
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DOI: https://doi.org/10.1295/polymj.35.255