Abstract
This paper examines the relationship between the conformation and solvent for the poly(dA)/s-SPG complex using circular dichroism in the 240–300 nm wavelength region. At the low temperature and low salt concentration, poly(dA) in the complex is estimated to take C2’-endo with a different torsional angle from the anti form. With increasing temperature, the circular dichroism (CD) spectrum starts to bear characteristics of C3’-endo and anti as a minority among the majority of C2’-endo and anti. When the salt concentration is increased, the spectrum becomes almost identical to that of the high temperature form under the non-salt condition. Therefore, addition of salt provides the identical effect to increasing temperature. D2O induces the same effect as decreasing temperature.
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Mizu, M., Koumoto, K., Kimura, T. et al. Polysaccharide–Polynucleotide Complexes Part 17. Solvent Effects on Conformational-Transition of Polydeoxyadenylic Acid in the Complexes with Schizophyllan. Polym J 35, 714–720 (2003). https://doi.org/10.1295/polymj.35.714
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DOI: https://doi.org/10.1295/polymj.35.714