Abstract
Chromobacterium sp. isolated from a river soil produced blend of a copolyester consisting of 3-hydroxybutanoic acid (3HB) and 3-hydroxypropionic acid (3HP) units and copolyester consisting of the saturated 3-hydroxyalkanoic acid (3HA) units of even carbon number C4 to C14 in addition to two unsaturated 3HA units [3-hydroxy-5-cis-dodecenate (3H5DD) and 3-hydroxy-7-cis-tetradecenoate (3H7TD)] when cultivated on 1,3-propanediol (1,3-PD) as the sole carbon source under nitrogen-free conditions. The copolyesters with 3HP unit have up to 6 mol% 3HP fractions, the number average molecular weight (Mn) with up to 4.4×105, the melting temperature (Tm) of 148–158°C and the glass transition temperature (Tg) of 2–4°C. In cotrast, the medium-chain-length polyhydroxyalkanoates (PHAMCL) with even carbon number of C6-C14 monomer units have ca. 60 mol% 3-hydroxydecanoate (3HD) fraction as the most predominant component and less than 4 mol% of 3H5DD and 3H7TD fractions as minor. The Mn, Tm and Tg of PHAMCL were ca. 1.2×105, 53°C and -45°C, respctively.
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A. J. Anderson and E. A. Dawes, Microbiol. Rev., 54, 450 (1990).
K. Sudesh, H. Abe, and Y. Doi, Prog. Polym. Sci., 25, 1503 (2000).
B. H. A. Rehm, T. A. Mitsky, and A. Steinbüchel, Appl. Environ. Microbiol., 67, 3102 (2001).
P. A. Holmes, Phys. Technol., 16, 32 (1985).
N. D. Miller and D. F. Williams, Biomaterials, 8, 129 (1987).
A. Timm and A. Steinbüchel, Appl. Environ. Microbiol., 56, 3360 (1990).
A. Steinbüchel, “Biomaterials," D. Byrom, Ed., Macmillan Publishers, Basingstoke, 1991, p 123.
Y. Doi, A. Segawa, and M. Kunioka, Int. J. Biol. Macromol., 12, 106 (1990).
H. Mitomo, N. Morishita, and Y. Doi, Macromolecules, 26, 5809 (1993).
N. Yoshie, H. Menju, H. Sato, and Y. Inoue, Macromolecules, 28, 6516 (1995).
E. Y. Lee, D. Jendrossek, A. Schirimer, C. Y. Choi, and A. Steinbüchel, Appl. Microbiol. Biotechnol., 42, 901 (1995).
M. Kato, T. Fukui, and Y. Doi, Bull. Chem. Soc. Jpn., 69, 515 (1996).
H. Matsusaki, S. Manji, K. Taguchi, M. Kato, T. Fukui, and Y. Doi, J. Bacteriol. 180, 6459 (1998).
H. Biebl, S. Marten, H. Hippe, and W.-D. Deckwer, Appl. Microbiol. Biotechnol., 36, 592 (1992).
P. L. Meredith, J. Polym. Sci., Part A: Polym. Chem., 38, 667 (2000).
U. Witt, R.-J. Müller, J. Augusta, and H. Widdecke, Macromol. Chem. Phys., 195, 793 (1994).
E. Ranucci, Y. Liu, M. S. Lindblad, and A.-C. Albertsson, Macromol. Rapid. Commun., 21, 680 (2000).
Y. Liu, E. Ranucci, M. S. Lindblad, and A.-C. Albertsson, J. Polym. Sci., Part A: Polym. Chem., 39, 2508 (2001).
R. Hugh and E. Leifson, J. Bacteriol., 66, 24 (1953).
API 20 NE that is test kit for identification of gram-negative bacteria was purchaced from bioMérieux-Vitek Japan, Ltd.
A. Steinbüchel, EI-M. Debzi, R. H. Marchessault, and A. Timm, Appl. Microbiol. Biotechnol. 39, 443 (1993).
H. Brandl, G. K. Hamer, R. W. Lenz, and R. C. Fuller, Appl. Environ. Microbiol., 54, 1977 (1988).
S. Nakamura, M. Kunioka, and Y. Doi, Macromol. Rep., A28, 15 (1991).
M. Hiramitsu and Y. Doi, Polymer, 34, 4782 (1993).
G. N. M. Huijiberts, G. Eggink, P. de Waard, G. W. Huisman, and B. Witholt, Appl. Environ. Microbiol., 58, 1949 (1992).
M. Kato, H. J. Bao, C.-K. Kang, T. Fukui, and Y. Doi, Appl. Microbiol. Biotechnol., 45, 363 (1996).
R. A. Gross, C. Demello., R. W. Lenz, H. Brandl, and R. C. Fuller, Macromolecules, 22, 1106 (1989).
Y. Doi, M. Kunioka, Y. Nakamura, and K. Soga, Macromolecules, 19, 2860 (1986).
Y. Saito and Y. Doi, Int. J. Biol. Macromol., 15, 287 (1993).
A. Steinbüchel, “Biotechnology", M, Roehr, Ed., VCH Publishers, New York, N.Y., 1996, p 403.
N. Hoffmann, A. Steinbüchel, and B. H. A. Rehm, FEMS Micro. Biol. Lett., 184, 253 (2000).
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Kimura, H., Yamamoto, T. & Iwakura, K. Biosynthesis of Polyhydroxyalkanoates from 1,3-Propanediol by Chromobacterium sp.. Polym J 34, 659–665 (2002). https://doi.org/10.1295/polymj.34.659
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DOI: https://doi.org/10.1295/polymj.34.659