Abstract
Tetrasaccharide Galβ1→4(GlcNAc)3 was synthesized from N,N′,N″-triacetylchitotriose (GlcNAC)3 and lactose using transglycosylation with a β-D-galactosidase from Bacillus circulans. The reducing terminal of Galβ1→4(GlcNAc)3 was oxidized and connected to p-vinylbenzylamine via amide linkage, and the resulting oligosaccharide-substituted styrene monomer was polymerized with the radical initiator, 2,2′-azobis(2-amidinopropane) dihydrochloride at 60°C. Glycopolystyrene was found to bind strongly with wheat germ agglutinin (WGA) and tomato (Lycopersicon esculentum) agglutinin (LEA) by inhibition of hemagglutination and double diffusion.
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Kobayashi, K., Kamiya, S., Matsuyama, M. et al. Synthesis and Functions of a Glycopolymer Carrying Galβ1→4(GlcNAc)3 Tetrasaccharide. Polym J 30, 653–658 (1998). https://doi.org/10.1295/polymj.30.653
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DOI: https://doi.org/10.1295/polymj.30.653