Abstract
The formation of a soluble complex of heparin and fibrinogen was observed in 0.05 M phosphate buffer at pH 7.0 in low ionic strength at 5°C. That is, the electrophoretic mobility, slightly depending on heparin concentrations, of the complex was faster than that of fibrinogen, and slower than that of heparin. Quantitative analysis on the complex was carried out by using the free boundary electrophoresis. In the soluble complex, heparin was regarded to be bound reversibly with fibrinogen and adsorbed to fibrinogen in the mode of the Freundlich adsorption isotherm. The dissociation constant between heparin and fibrinogen was evaluated to be 7.4×10−4 M, so that the interaction in the soluble complex with fibrinogen was considered to be too loose to contribute toward the anticoagulant activity of heparin.
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Nakanishi, E., Sato, H. & Nakajima, A. Electrophoretic Study on a Soluble Complex of Heparin and Fibrinogen. Polym J 22, 510–517 (1990). https://doi.org/10.1295/polymj.22.510
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DOI: https://doi.org/10.1295/polymj.22.510