Abstract
Raman spectra were measured for copolymers of L-alanine and glycine and those of L-alanine and L-phenylalanine. The L-alanine-glycine copolypeptides with a glycine residue content of 3—21 mol% take the α-helical conformation. On incorporation of the glycine residue at more than 30%, the backbone structure changes partly from the α-helix to the β-form. The L-alanine–L-phenylalanine copolypeptides with the L-phenylalanine content of 12—92% take the α-helical structure. The following Raman bands reflect the α-helix or the β-form structure of the component amino acid residues; the bands near 1308, 1108, 530 and 378 cm−1 are characteristic of the L-alanine residue with the α-helical structure, the band near 1094 cm−1 of the L-alanine resdue with the β-form, the band in the 470—480 cm−1 region of the L-phenylalanine residue with the α-helix, and the band near 490 cm−1 of the L-phenylalanine with the β-form.
Similar content being viewed by others
Article PDF
References
B. G. Frushour and J. L. Koenig, “Advances in Infrared and Raman Spectroscopy,” Vol. 1, R. J. H, Clark and R. E. Hester, Ed., Heyden, 1975, p 35.
T. Miyazawa, K. Fukushima, S. Sugano, and Y. Masuda, “Conformations of Biopolymers,” Vol. II, G. N. Ramachandran, Ed., Academic Press., New York., N. Y., 1967, p 557.
K. Itoh, H. Katabuchi, and T. Shimanouchi, Nature New Biology, 239, 42 (1972).
K. Itoh and H. Katabuchi, Biopolymers, 12, 921 (1973).
K. Itoh, H. Hinomoto, and T. Shimanouchi, Biopolymers, 13, 307 (1978).
G. D. Fasman, K. Itoh,C. S. Liu,and R. C. Rord, Biopolymers, 17, 125 (1978).
M. Oya, K. Uno, andY. Iwakura, J. Polym. Sci., A-1, 10, 613 (1972).
M. Oya and T. Takahashi, J. Polym. Sci., Polym. Chem. Ed., 20, 529 (1982).
T. Miyazawa, J. Chem. Phys., 32, 1647 (1960).
T. Miyazawa and E. R. Blout, J. Am. Chem. Soc., 83, 712 (1961).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Itoh, K., Oya, M. Raman Spectra and Conformations of Copolypeptides Containing Glycine, L-Alanine, and L-Phenylalanine Residues. Polym J 18, 837–842 (1986). https://doi.org/10.1295/polymj.18.837
Issue Date:
DOI: https://doi.org/10.1295/polymj.18.837