Abstract
An attempt was made to establish correlations among molecular characteristics, blood anticoagulant activity, and acute toxicity of sodium cellulose sulfate (NaCS). For this purpose, 25 NaCS samples ranging in number-average molecular weights Mn from 800 to 36.8×104 and in total degree of substitutions ‹‹F›› from 0.5 to 2.75 were prepared. The distribution of substituent groups on three carbon positions (C2, C3, and C6) in a glucopyranose unit ‹‹fk›› (k=2, 3, and 6) of NaCS was determined by the NMR method. The anticoagulant activity of NaCS to the whole blood was evaluated by the methods of Lee-White, Imai, and the Commentary of Japanese Pharmacopoeia. Acute toxicity, as measured by LD50 (vein injection), of NaCS for rats was determined by the Probit method. Inhibitory action of NaCS on blood coagulation was estimated using a coagulation factor deficient substrate plasma and a sample plasma from normal blood. It was found that the sum ‹‹f2››+‹‹f3›› is a predominant factor for the anticoagulant activity of NaCS, but Mn is only a minor factor. LD50 of NaCS was found to be intricatedly influenced by ‹‹f2››+‹‹f3›› and Mn. The inhibitory action of NaCS towards coagulation factor VIII was found to be much more intensive than heparin. An anatomical study showed that after injection of NaCS, no abnormality occurred in live rats but a state of anemia appeared in the liver of dead rats and congestive and blood shots occurred in their lungs.
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Kamide, K., Okajima, K., Matsui, T. et al. Roles of Molecular Characteristics in Blood Anticoagulant Activity and Acute Toxicity of Sodium Cellulose Sulfate. Polym J 15, 309–321 (1983). https://doi.org/10.1295/polymj.15.309
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DOI: https://doi.org/10.1295/polymj.15.309
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